Agriculture and food

Microarray technology for agriculture and food application

in Agriculture and food

Microarray technology allows high-throughput screening and analysis. BIOTEC Microarray Laboratory is equipped with technologies to fabricate array chips inhouse as well as applying them in various aspects of research and development. To understand fundamental biology of black tiger shrimp (Penaeus monodon), the first version of cDNA microarray has been constructed to study the reproduction and nutrigenomics of this economically important animal.

Shrimp

in Agriculture and food

Thailand is a leader in cultivated shrimp production and it has gained a competitive advantage through higher farm productivity and better disease control. Major export markets for Thai shrimp are the USA and Japan.

Domestication of black tiger shrimp is critical to help overcome the problems arising from the use of wild shrimp and domesticated stocks. It also allows for the selection of important traits, such as the fast-growing or disease-resistance strains in this species. Raising domesticated specific pathogen-free (SPF) black tiger shrimp broodstock is one of the main research priorities and it involves both the government and the private sector.

Marker-assisted selection

in Agriculture and food

In Thailand, marker-assisted selection has been used in breeding programs for tomatoes, to select for plants showing resistance to bacterial wilt as well as nematode, tobacco mosaic virus, fusarium race 2, late blight and powdery mildew. Resistant lines are currently being evaluated in multi-location fields by private companies.

Downy mildew is one of the most destructive diseases found in maize in Thailand. Researchers have been using molecular techniques, such as Simple Sequence Repeat (SSR), to define marker positions that link to the QTLs of the disease, which are then used in marker-assisted selection. Eight novel QTLs for resistance have been found.

Sugarcane

in Agriculture and food

Thailand produces about 73 million tons of sugarcane annually. Strategies for improve productivity include better farm management, irrigation and improved varieties. Each variety has specific characteristics suitable for a particular planting area and environment.

Research on improving sugarcane for food focuses on high biomass (increasing high productivity per unit area), high sugar content (13 CCS), disease resistance, ratooning ability (more than 2 crops), while for energy cane the focus is on high fiber (raw material for electricity) and high sugar content (raw material for ethanol production). For forage cane the focus is on digestibility (soft fiber) and higher nutritional value for animal feed.

Seed

in Agriculture and food

The seed industry is considered to be an essential driving force in the modernization of Thailand’s agriculture sector and modernization is focused on greater value creation of existing agricultural commodities, where Thailand is already a world leader in production. Due to a favorable climate and fertile farmland, Thailand is currently the seed production and manufacturing base for many international seed conglomerates. In 2006, there were over 100 local and foreign seed companies in operation, employing more than 28,000 families and contributing over US$420 million (not including local demand of over US$300 million) to the Thai economy.

Rice

in Agriculture and food

Thailand is the leading exporter of rice and is well known for its fragrant or jasmine varieties, popular in many countries. The premium quality variety is known as KDML 105 and Thailand already has introgression lines with submergence tolerance, bacterial leaf blight resistance, leaf blast resistance, brown plant hopper resistance, drought and salt tolerance. Future efforts will look into putting these traits together by gene pyramiding via marker-assisted selection.

Plant transformation

in Agriculture and food

An early application of this technique in Thailand’s agricultural scene was the development of transgenic tomato plants resistant to the tomato yellow leaf curl virus. BIOTEC’s Plant Genetic Engineering Unit at Kasetsart University has successfully created tomato plants that carry the coat protein gene of the tomato yellow leaf curl virus, to help control this economically-damaging viral disease.

The same approach was taken to develop transgenic papaya and pepper that are resistant to the papaya ringspot virus (PRSV) and transgenic chili that is resistant to the vein-banding mottle virus.

Maintaining the lead

in Agriculture and food

Thailand is the world leader in the production and export of many agricultural products, including rice, cassava, sugar and rubber. It is the 14th largest agricultural and food exporter in the world (or the 5th largest if all the European countries are considered as a single community). The food and agriculture industry is therefore an important contributor to the economy. However, to maintain its lead and share in the world market, the industry must overcome several challenges including productivity, quality and food safety and one of the biggest challenges facing Thailand is its continued ability to export agricultural commodities to the USA and the EU.

Cassava and starch

in Agriculture and food

Cassava is one of the most important commercial crops in Thailand. It is exported mainly to Europe in the form of chips for animal feed. The starch produced from cassava has wider utilization in both food and non-food applications. Industrial utilization of cassava starch has now expanded from being primarily used in paper, textile and plywood industries to the production of biofuel and biodegradable polymers. There are currently over 70 factories producing around 2 million tons of starch per annum. For the domestic market, the largest proportions of native starch go to the industrial production of food flavor enhancers, such as monosodium glutamate, lactic acid and amino acid production for feed supplement, such as lysine.

Biological control

in Agriculture and food

In addition to the obvious dangers from overuse of pesticides, the growing popularity of organic produce among consumers around the world represents an opportunity for Thai farmers, who are well positioned to supply fresh organic produce, especially fruit and vegetables, internationally.

While field application is still limited, some successful bio-control programs include:

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